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UCL Great Ormond Street Institute of Child Health (D13)

UCL Great Ormond Street Institute of Child Health (D13)

D13 Image Processing & Analysis

D13 Image Processing & Analysis with ImageJ / Fiji 08/02/2022

Description

The course is spread over 3 days. It introduces image analysis with the free software ImageJ / Fiji.

Content: lectures, demos, hands on sessions and exercises. Homework will be included.

Format: online via Zoom. You must install zoom, do not use the web browser version.

 

Day 1) 10am - 5pm (breaks for Lunch and tea/coffee)

Understanding digital images. Bit depth, brightness, colours & pixels. ImageJ / Fiji basics, getting to grips with the software. Image presentation (generating figures for publication). ROIs and simple measurements.

 

Day 2) 10am - 5pm (breaks for Lunch and tea/coffee)

Image analysis. Pre-processing (Filters, background), thresholding for feature identification and counting. Alternative segmentation strategies. 2D 3D and timeseries. Identifying double / triple stained cells, (and why this isn't colocalisation).

 

Day 3) 10am - 5pm (breaks for Lunch and tea/coffee)

ImageJ / Fiji macros. Recording and writing custom scripts to automate image analysis.

 

The course is presented via zoom. You must have a PC with Fiji and Zoom installed.

https://www.ucl.ac.uk/ich/core-scientific-facilities-centres/confocal-microscopy

Attendee CategoryCost   
1) ICH Imaging Facility Users.£0.00[Read More]
2) ICH Non-imaging Facility Users.£50.00[Read More]
3) UCL Academic.£75.00[Read More]
4) External Academic.£120.00[Read More]
5) Non-Academic.£200.00[Read More]

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