Event Location

Contact

For all queries in regards to this Course please contact the following :-

Rachel Vernazza

mbi-training@ucl.ac.uk 

PLEASE ONLY CONTACT THE ONLINE STORE DIRECTLY IF YOU ARE EXPERIENCING PROBLEMS WITH YOUR DEBIT/CREDIT CARD PAYMENT, FOR ALL OTHER QUERIES RELATING TO THIS COURSE, INCLUDING CANCELLATIONS THESE SHOULD BE DIRECTED TO THE CONTACT DETAILS ABOVE.

More Information

Tutorials for SOPs, data analysis, and troubleshooting, will be supported by a series of introductory and expert lectures that will enable you to:

• Apply basic aseptic techniques relevant to the culture and passaging of mammalian cells.
• Perform procedures for preserving mammalian cells and retrieving those from frozen (liquid nitrogen) storage for subsequent cultivation.
• Quantify cell numbers using a manual haemocytometer and an automated cell counter.
• Assess the viability of mammalian cells using conventional Trypan blue exclusion assay and fluorescent dyes for labelling live and dead cells.
• Identify the typical morphologies of mammalian cells and examine cell activity by immunocytochemistry and image analysis software.
• Recognise conventional cell culture equipment including Carbon Dioxide incubators and Class II Biological Safety Cabinets (BSCs) and apply that equipment for the culture of mammalian cells.
• Apply tissue culture and analytical laboratory safety procedures and recognise the importance of those protocols to safeguard laboratory personnel.
• Apply biological waste management protocols appropriate for all aspects of mammalian cell culture and analysis.