This course is designed to provide delegates with a practical understanding of the set-up, inoculation and operation of lab and small pilot scale, single-use bioreactors for the culture of different types of mammalian cell used for biotherapeutic and vaccine production.

The course will provide delegates opportunities to sub-culture adherent HEK cells and suspension CHO cells from shake flask sub-culture through to laboratory scale bioreactors including a single-use, stirred bioreactor system (10L scale) and an iCellis® Fixed-Bed Bioreactor.

Delegates will get hands-on experience with mammalian cell sub-culture prior to familiarisation with standard operating procedures (SOPs) for typical mammalian bioprocesses. Delegates will also be provided with hands-on training in aseptic technique, seed train preparation and maintenance, and bioreactor seeding, sampling, and operation. Delegates will also be given key insight into operational methods/alternatives and general design considerations for mammalian bioprocesses.

In addition, delegates will learn industrially relevant analytical techniques including cell counting, viability and metabolite analysis.